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Isolation and characterization of a Bacillus licheniformis strain (8). "Seaweed could fight tooth decay scientists". [12] Amylases are also synthesized by B. licheniformis and used for industrial purposes. All rights reserved. Strictly aerobic. Part of colony demonstrating irregular form, lobate margin. (PDF) Colony Morphology | Psilocybe Spp. - Academia.edu It may have a putative impact on protease production and serve as target for strain improvement.[4]. Recently discovered filament-forming proteins run along the longer axis of rod-shaped cells and push original and replicated DNA to each end during cell division. Where is Bacillus species found? species and related bacteria in human faeces. This is the first report of an Alternaria toxin-degrading . Endospores allow the organism to survive in a variety of harsh environments (high salt concentrations, for example) until conditions are more favorable, when it returns to a vegetative state. (4, 6, 7, and 8). 2. Its use as an expression vector for the production of enzymes and . Irregular form, undulate margin. The sample was obtained from a floor swab and incubated at 37C for 48 hours. Lab tests have confirmed the enzyme's ability to break up and remove bacteria present in plaque, and thus prevent the build up of plaque. It is found on bird feathers, especially chest and back plumage, and most often in ground-dwelling birds (like sparrows) and aquatic species (like ducks ). The death kinetics for B. licheniformis ATCC 14580 spores in cloudberry jam was examined under ohmic heat inactivation and conventional heat inactivation. Weakly umbonate elevation. 1965 February; 89(2): 415420. morphology is variable, and may give the appearance of a mixed culture. The inhibitory effect of Ukrainian honey on probiotic bacteria 6346. The main objective of this study is to observe the phenol degradation performance by free and immobilized Pseudomonas putida (P. putida) in batch and continuous reactors, respectively. Priestia aryabhattai B8W22 | Type strain | DSM 21047, JCM 13839, MTCC A colony of Bacillus subtilis survived on the outside of a NASA satellite for six years. Then, using confocal microscopy, we localized the GFP-tagged strain within plant tissues of inoculated grapevine Glera cuttings. cultivated on trypticase soy agar. Weakly irregular form. 2006 May. Unknown isolate cultivated on trypticase soy agar. Bacillus subtilis PabrB-gfp after 14 days viewed at high magnification. Circular form, entire margin. 6346. supplementation on the apparent ileal Gerardi, M. (2016). . Characteristics of the bacterial colony on agar slants, Characteristic of bacteria in nutrient broth, Microorganisms that can grow on a nutrient agar plate, Factors affecting the colony morphology of bacteria, Shape A bacterial colony can manifest in various, Staphylococcus irregularly arranged in clusters/grape-like. Non-typical Bacillus strains may be very small and spore formation often fails to occur. Find common criteria that are used to characterize bacterial growth; Colony shape Size of the bacterial colony Appearance of the colony surface Consistency/Texture The sample was grown at 4C for one month. Catalase-positive. I realize that the strains causing these problems were wild strains, not strains studied to be probiotics. 52. Gram-positive bacilli are rod-shaped, spore-producing species that can survive in extremely harsh environments for long periods. FIG. Circular form. Buiuc D., Negut M. , 1999. Various organisms can grow on solid media. A characteristic strawberry-like odor is produced by some strains. FIG. The optimal temperature for enzyme secretion is 37C. Kurthia sp. PDF Identification Of Bacillus Species - Webmail.gestudy.byu.edu But, although similar to B. subtilis, they differ in the amount and location of prophages, transposable elements, extracellular enzymes, and secondary metabolic pathway operons. Also, cultures of B. licheniformis are made to retain its protease, which is in turn used in laundry detergent. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 43: Unknown isolate morphology (Enlarged view). Lactobacillus plantarum cultivated on lactobacillus agar. Addition of this enzyme to toothpastes, mouthwash, etc. Note the lemon-yellow colonies characteristic of this organism. Colonies are both round and irregular in shape, with irregular (undulate, fimbriate) margins. Frontiers | Genomic Analysis of Bacillus licheniformis CBA7126 Isolated B. licheniformis is naturally competent for genetic transformation. (D. Barrie Johnson, University of Wales, UK), Figure 32: Thiomonas-like bacteria morphology (Enlarged view). Bacillus licheniformis is a hardy probiotic which is commonly combined with other Bacillus bacteria for optimal probiotic effects. Unlike other bacilli which are predominately aerobic, B. licheniformis is a facultative anaerobe, which explains it's ability to grow in additional ecological niches and environments. "Bacillus Subtilis." Biology Dictionary. Irregular form, lobate margin. Colonies are described based on size, shape, texture, elevation, pigmentation, and effect on growth medium. Iron-oxidizing Thiomonas-like bacteria isolated from mine waters. It has a cell wall that is made of a complex. B. licheniformis is used to make the antibiotic Bacitracin. Bacillus Licheniformis - an overview | ScienceDirect Topics Batch experiments were evaluated to determine the maximum specific growth rate, saturation constant, inhibition constant . In vivo bioassays were used to investigate the effects of each gut bacterium namely, Fructobacillus fructosus (T1), Proteus mirabilis (T2), Bacillus licheniformis (T3), Lactobacillus kunkeei (T4), Bacillus subtilis (T5), Enterobacter kobei (T6), and Morganella morganii . (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 46: Streak plate isolation of Mycobacterium smegmatis on trypticase soy agar. (7) Wecke T, Veith B, Ehrenreich A, Mascher T. Cell envelope stress response in Bacillus licheniformis: integrating comparative genomics, transcriptional profiling, and regulon mining to decipher a complex regulatory network. The surface of B. licheniformis colonies are often rough and wrinkled, with "licheniform", or hair-like growths. The bacteria release exoenzymes that hydrolyze the agar. 2003 Apr;69(4):2321-9. The initial sample was obtained from a swab taken from the bottom of a shoe and incubated at 37 C for 48 hours. Biotechnol. (Richard A. Robison, Gable Moffitt, Neal Thomson, and Marissa Cohen, Brigham Young University, Provo, UT) Figure 21: Unknown isolate morphology (Enlarged view). Can J Microbiol 51: 191 196. Find out more here. 26. (3) Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Jorgensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M. As mentioned, Bacillus licheniformis is commonly found on bird feathers; by fermentation with B. licheniformis, the large amounts of non-digestible proteins found in the feathers can turn into a feather meal for livestock. Putative antibiotic resistance genes present in extant Bacillus - PLOS Bacillus subtilis is a Gram-positive, rod-shaped bacterium that is the type species of the genus Bacillus, commonly used as a model organism to describe the structure of different species of the genus. It is also resistant to some commonly used antibiotics, including oxacillin and nafcillin. It is a gram-positive, mesophilic bacterium. websites owned and operated by ASM ("ASM Web Sites") and other sources. FIG. What is a colony? Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus. The strain was identified as Bacillus licheniformis GL174 and its endophytic attitude was validated by plating surface-sterilized inoculated cuttings. Irregular form, lobate margin. Plasmid For Bacillus Expressing Fluorescent Reporter Genes It was found in the fetal stomach contents showing it was able to enter the bloodstream of the adult and cross the placenta to a bovine fetus. Current research try to find the "death kinetics" (2) of these bacteria. 25. (1) Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Jorgensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M. The majority of isolates fell into the Bacillus Bergey's Group A together with Bacillus subtilis and Bacillus licheniformis. This present paper explores the ability. The ability of B. licheniformis to form endospores allows it to survive in the harsh environments required to manufacture industrial enzymes, chemicals, and antibiotics. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 42: Serratia marcescens morphology (Enlarged view). Circular form, entire margin. (4). (Richard A. Robison, Gable Moffitt, Neal Thomson, and Marissa Cohen, Brigham Young University, Provo, UT), Figure 8: Streptococcus pneumoniae morphology (Enlarged view). Your email address will not be published. The bacterium was subcultured by cutting a chunk of the agar containing the organism and placing it in the middle of a TSA plate then incubating for 1 week at 37 C. this information and the choices you have about how we use such information. Umbonate elevation. PDF Attachment I-- Bacillus Licheniformis I. Introduction - Us Epa Bacillus licheniformis is a nonpathogenic soil organism. Bacillus cereus is an aerobic spore-forming bacterium that is commonly found in soil, on vegetables, and in many raw and processed foods. Optimal growth temperature is 20-37 C. [13][14], B. licheniformis is used as a probiotic in animal feed, where isolates have been shown to prevent disease and promote growth as well as being commercially available. PDF Assessing the inhibitory activity of culture supernatants against Bacillus subtilis PspoIIA-gfp viewed at low magnification. The effects of temperature(30-40C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat,rice and corn starches) and substrate concentration (5-30 g/L) on the . Bacillus Flashcards | Quizlet (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 35: Nocardia asteroides morphology (Enlarged view). 37. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 12: Unknown isolate morphology (Enlarged view). Tratat de Microbiologie Clinica, Editura Medicala, Bucuresti. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 6: Lactobacillus plantarum morphology (Enlarged view). It is also a facultative anaerobe. Close-up view of the edges of an unknown bacterium on trypticase soy agar (TSA) exhibiting spreading and rhizoid growth. (Tasha L. Sturm, Cabrillo College, Aptos, CA), Figure 50: Serratia marcescens colonial morphology. Bacillus licheniformis is a bacterium that is commonly found in soil and bird feathers. Circular form, convex elevation, entire margin. 2006 Nov;188(21):7500-11. Irregular form, weakly undulate margin. ", Biologydictionary.net Editors. Irregular form, lobate margin. It is a gram-positive, mesophilic bacterium. The pigment is opaque white-beige with a mucoid surface towards the center of the colony and a matte surface towards the edges. Biotechnological applications of Bacillus licheniformis [20], Below is a list of differential techniques and results that can help to identify Bacillus licheniformis from other bacteria and Bacillus species.[27]. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 41: Kurthia morphology (Enlarged view). Circular form, convex elevation, entire margin. Elevation it pertains to the cross-sectional shape of the colony or side view of the colony such as: Margin It pertains to the magnified shape of the colonys edge which includes: Opacity it pertains to the characteristics of the colony such as: Image 5: Images of the bacterial colony with a varying degree of pigmentation. milk pasteurization. 23. (2012). Morphological, physiological and biochemical characteristics of these strains were studied by optimizing their growth conditions such as pH, NaCl and temperature. microbial colonies that showed mucus morphology were streaked on TSA plates to obtain pure cultures. Probiotic Potential of Bacillus licheniformis and Bacillus pumilus For which exoenzyme does this test? Filamentous form. (Richard A. Robison, Gable Moffitt, Neal Thomson, and Marissa Cohen, Brigham Young University, Provo, UT), Figure 20: Bacillus thuringiensis morphology (Enlarged view). FIG. FIG. The desirable properties of this protease are its prevention of clothes shrinkage and fading colors due to its capability to be used at lower temperatures. Under good conditions, the spores will germinate and produce vegetative cells. sparrows)and on the water (i.e. Sheep blood agar (SBA) Colonies are nonhemolytic, flat or slightly convex with irregular edges and ground-glass appearance. Irregular form, undulate margin. FIG. Color is whitish and may become brown. B. licheniformis has proved itself as a multipurpose organism and has gained popularity along with B. subtilis. Description of the Genus - Bacillus - Hardy Diagnostics FIG. It is particularly effective against Enterococcus faecalis, Enterobacter aerogenes, Streptococcus pyogenes, and Shigella sonnei. This protease is a desired ingredient in laundry detergent due to its ability to be used in low temperatures, which prevents shrinkage and fading colors. 4959. The pathogenesis of Bacillus licheniformis, based on experimental studies, suggests there is localization in the placentomes after bacteremia. the morphology of B. subtilis, B. licheniformis, . B. licheniformis is a motile organism capable of fermenting sugars (lactose, glucose, mannitol) and excreting useful extracellular enzymes including but not limited to: catalase, casease, urease, deaminase, protease, and lipase. FIG. FIG. Genome Biol. This bacterium, although detrimental, can be modified to become useful. Bacillus licheniformis and Bacillus subtilis are closely related species . FIG. Since it can grow in alkaline conditions, it produces a protease that can survive at high pH levels. These bacteria are commonly known to cause food poisoning and food spoilage. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 39: Lactobacillus plantarum morphology (Enlarged view). Ecologists are looking for signs of association between the plumage feathers and B. licheniformis activity. Proteases are often included in detergents, and amylases in the desizing of textiles and sizing of papers. Corynebacterium xerosis cultivated on trypticase soy agar. (3). Practice all cards Practice all cards Practice all cards done loading. Optimal growth of B. licheniformis occurs around 50C, but the organism can survive at much higher/lower temperatures for extended periods because it is spore-forming. Unknown isolates cultivated on trypticase soy agar. Bacillus subtilis morphology describes rod-shaped, Gram-positive bacteria that show up on both positive and negative Gram stain techniques. Isolation and Molecular Characterization of Antibiotic Producing . FIG. Washington, DC 20036, 2023. It causes bread spoilage, or more specifically, a condition called "ropy bread" (1). High expression of Bacillus licheniformis alpha-amylase with a Bacillus secretion vector. It is also often found on feathers of ground-dwelling and aquatic species of birds. The results of this carbohydrate fermentation test read A. AG, AG, K B . Bacillus licheniformis is a Gram positive spore-forming bacterial species of high biotechnological interest with numerous present and potential uses, including the production of bioactive compounds that are applied in a wide range of fields, such as aquaculture, agriculture, food, biomedicine, and pharmaceutical industries. (Bobbi Pritt, Mayo Clinic, Rochester, MN), Figure 11: Unknown isolate morphology (Enlarged view). B. licheniformis is also known to cause food poisoning in humans; especially high in contamination rates are products such as raw milk, dairy, vegetables, processed baby foods, and cooked meats. FIG. Information on morphological and physiological properties Morphology [Ref. A bacterial colony is limited by. Novozymes Biofungicide Green Releaf contains B. licheniformis strain SB3086 as an active main ingredient. A white base of hyphae surrounds green and yellow grainy spore formation in the center of the colony. 8: Bacterial Colony Morphology - Biology LibreTexts (Volker Briken, University of Maryland, College Park, MD), Figure 34: Unknown isolate morphology (Enlarged view). There is current research on B. licheniformis (strain SB3086) and its effects as a microbial fungicide. There are often comma shaped projections from the colony edge producing a "Medusa-head" colony. [ 55 ] evaluated the effects of a combination of enzymes with probiotics (3 Bacillus spp.) Wastewater Bioaugmentation and Biostimulation. Previous studies had found that B. licheniformis could produce a variety of biologically active substances, such as digestive enzymes, lysozyme, bacteriocin, and antibacterial peptides, which promote animal performance by improving feed digestibility, stimulating the development of immune system, enhancing intestinal mucosal barrier function, The probiotic effects of seven newly isolated gut bacteria, from the indegenous honey bees of Saudi Arabia were investigated. 18. Filamentous form, weakly undulate margin. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 14: Pasteurella multocida morphology (Enlarged view). Although B. licheniformis often occurs in high numbers in these foods, it's presence is not usually regulated in contrast to B. cereus, which is credited with most food poisoning incidents by the Bacillus species. FIG. 29. FIG. FIG. This text tells us that up to 5% of the B. subtilis genome is dedicated to the production of antimicrobial compounds (AMCs). [18] Natural genetic transformation is a sexual process involving DNA transfer from one bacterium to another through the intervening medium, and the integration of the donor sequence into the recipient genome by homologous recombination. 35. Bacillus anthracis, the etiological agent of anthrax, is a gram-positive, spore-forming rod, with colonies exhibiting a unique ground-glass appearance, and lacking hemolysis and motility.In addition to these phenotypes, several others traits are characteristic of B. anthracis such as susceptibility to gamma phage, the presence of two virulence plasmids (pX01 and pX02), and specific . Lactobacillus plantarum cultivated on lactobacillus agar. There is also research about the possibility that B. licheniformis causes changes in color in birds' feathers; this will provide information on the evolution of molting. 20. The protease has an optimum pH at around 9 and 10, which is desirable since it can remove protein-comprised dirt in clothes. A fluorescence reporter plasmid system for enabling a Bacillus strain to fluoresce, wherein the Bacillus strain fluoresces in its dormant endospore state and/or in its metabolically active vegetative state, and wherein the plasmid system is designed to function extra-chromosomally. Streak plate isolation of Serratia marcescens on trypticase soy agar (TSA) incubated at 37oC for 24 hours then at room temperature for 24 hours to allow for temperature-sensitive pigment production. Its optimal growth temperature is around 50C, though it can survive at much higher temperatures. Identification and characterization of clinical Bacillus spp. isolates The bacterium was subcultured by cutting a chunk of the agar containing the organism and placing it in the middle of a TSA plate then incubating for 1 week at 37 C. A colony is defined as a visible mass of microorganisms all originating from a single mother cell, therefore a colony constitutes a clone of bacteria all genetically alike. Microorganisms | Free Full-Text | Bacillus licheniformis and Bacillus The bacterium grew to a fractal colony through the diffusion-limited aggregation process, a round colony reminiscent of the Eden model, a colony with a straight and densely branched structure similar to the dense branching morphology, a colony spreading without any openings, and a colony with concentric rings, on plates with various agar and Staphylococcus aureus cultivated on trypticase soy agar. This system uses DNA-based diagnostic tool to monitor fermentation processes such as the production of enzymes for Henkel laundry detergents. The colony morphology of Bacillus spp. Cotyledonary necrosis and suppurative inflammation occur with fetal infection resulting from fetal bacteremia or ingestion of contaminated amniotic fluid. (Tasha L. Sturm, Cabrillo College, Aptos, CA), Figure 48: Colonial morphology of Escherichia coli and Micrococcus luteus. Bacillus thuringiensis cultivated on 5% sheep blood agar. (3), The chromosome of B. licheniformis has large regions that are similar to Bacillus subtilis and Bacillus halodurans. General characteristics: Gram-positive, long, spore-forming rods with a diameter of < 0.9 m. FIG. Morphological and molecular based identification of - ScienceDirect confidence and trust with respect to the information we collect from you on Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus are some of the strains that are associated with food spoilage and with a wide range of different clinical conditions (Turnbull, 1997). White hyphal growth can be seen throughout the colony with a slight pink coloration in the center. FIG. The cell wall of Bacillus licheniformis is composed of mucopeptide, which is made up of linear chains of alternating amino sugars and short peptide chains of 3-5 amino acids. A. Bacillus licheniformis B. Bacillus megaterium C. Bacillus globigii D. Bacillus mycoides 39. 16. In the present study, CotA laccase was cloned from Bacillus licheniformis ZOM-1 and expressed in Escherichia coli. ruled out continue with routine identification major characteristics of bacillus anthracis gram stain morphology large gram positive rods spores may be . FIG. They include the following: Image 11: A colony of yeast on the agar plate. cultivated on trypticase soy agar. Irregular form, undulate margin. As these bacteria extract carbon and produce heat, the plastic polymers slowly degrade. The pigment is opaque white-beige with a mucoid surface over most of the colony. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 18: Kurthia morphology (Enlarged view). It is a visible mass of microorganism that originates from a single mother cell. Isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. Morphology of Bacillus subtilis. Bacillus licheniformis is a ubiquitous bacterium commonly found in the soil and wasted organic material, and is used extensively for the production of industrial enzymes, such as amylase and protease (Waldeck et al. The genus Bacillus covered a great diversity of strains and some of them are strictly aerobic, while others are facultative anaerobic. B. licheniformis exhibits antimicrobial activity against both Escherichia coli and Staphylococcus aureus. [13][15][16] Some isolates have also been found to be probiotic in humans (and are also commercially available),[13][17] but it's been mentioned that clinical trials have not been performed on many of them yet. Salkinoja-Salonen S., Vuorio R., Andersson M.A., Kmpfer P., Andersson M.C., Honkanen-Buzalski T., and Scoging A.C. Poovendran, P., Kalaigandhi, V., KamalaseKanan, V., Jamuna rani, E., Poongunran, E. Ramnani P, Singh R & Gupta R (2005) Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation. 19. (Tasha Sturm, Cabrillo College, Aptos, CA), Figure 58: An unknown airborne environmental isolate on Mueller-Hinton agar. This reaction also occurs in Gram-negative bacteria; however, the significantly lower levels of peptidoglycan mean that cell samples do not remain purple when a pink counter-stain (safranin) is added. Bacillus subtilis PspoIIA-gfp viewed at low magnification. Growth temperature from 15 C to 50-55 C. Weakly irregular form. Wastewater must have its pH normalized, have lower chemical oxygen demand (COD) and total suspended solids (TSS) concentrations, and be free of excess chloride. II. 31. Lancaster (PA), DEStech Publications. (Remco Kort, TNO, Netherlands), Figure 26: Bacillus subtilis morphology (Enlarged view). Bacillus thuringiensis cultivated on 5% sheep blood agar. (2) Pereira R., Martins J., Mateus C., Teixeira J. Bacillus pumilus - SlideShare "Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." (4) Salkinoja-Salonen S., Vuorio R., Andersson M.A., Kmpfer P., Andersson M.C., Honkanen-Buzalski T., and Scoging A.C. Toxigenic Strains of Bacillus licheniformis Related to Food Poisoning.Appl Environ Microbiol. FIG. Both require the presence of a zinc cofactor to be effective. Currently there are many electrical techniques for food processing, one such example is Ohmic heating. It can cause ophthalmitis, which is the inflammation of the eye. Image 10: Growth of bacteria colony in nutrient broth. 8. Genome Biol. 4. Irregular form, lobate margin. The Bacillus subtilis strain showed various colony growth patterns on the agar plate. (4). Bacillus subtilis can also degrade polyethylene (plastic polymers). Streptococcus salivarius cultivated onMitis-salivarius agar. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 30: Bacillus licheniformis morphology (Enlarged view). Because it is capable of forming endospores that can be easily disseminated, B. licheniformis can be isolated from a variety of places, though it is mainly associated with plant materials. It is spore-forming under harsh conditions and closely related to the widely studied B. subtilis. Non-pathogenic forms are often used in the biotechnology sector, including Bacillus subtilis. Without the support of synthetic chemicals, B. subtilis is not the fastest strain it reduces dry-weight plastic by around 1.75% over a term of 30 days. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 3: Streptomyces albus morphology (Enlarged view). B. licheniformis is a rod-shaped, gram positive motile bacterium. Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. CotA laccase from Bacillus licheniformis ZOM-1 effectively degrades Unknown isolate cultivated on trypticase soy agar. Arshine Questions and Answers about Bacillus Licheniformis.docx . Bacillus - Logan - Major Reference Works - Wiley Online Library Microbiology. FIG. Convex elevation. The pigment is opaque white-beige with a mucoid surface towards the center of the colony and a matte surface towards the edges. It is a rod shaped, endospore bearing bacteria and belong to the family Firmicutes.